With an estimated 10,000 new cases of invasive cervical (and 40,000 non-invasive), this cancer is common among women. The American Cancer Society estimates that about 3,710 of those women with cervical cancer will die from the disease this year. Since there is a 100 percent survival rate for women who are diagnosed early enough, 3,700 is far too high a number. Human papillomavirus (HPV) has been implicated as a major risk factor for cervical cancer. HPV has a cancerous effect on the cell cycle. In order to live and reproduce, the virus induces synthesis of DNA and moves them to the activated G1 phase of the cell cycle. By disrupting regulatory and tumor suppressor proteins, HPV oncogenes cause the cell to undergo uncontrolled cellular proliferation and malignancy. Curcumin, the major yellow pigment of turmeric obtained from the curcuma longa linn plant, has been demonstrated as an effective anti-inflammatory and chemo-preventive substance. We believe that treatment with this substance will inhibit growth of cervical cancer cells. The purpose of this study is to assess the effect of curcumin on the growth of HeLa cervical cancer cells. Following cell culture and protein extraction, 2-dimensional electrophoresis was used to separate proteins based on isoelectric point then molecular weight. Gels were stained and analyzed using PDQuest software. Unique protein spots within each sample group were identified, extracted, trypsin digested and analyzed for identification of differentially expressed proteins.