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PULSe Home > Faculty Members A-C > Kimberly Buhman
Kimberly K. Buhman
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Assistant Professor, Department of Foods and Nutrition
Ph.D., Nutritional Biochemistry, Purdue University, 1999
Contact Info:
kbuhman@purdue.edu
765-496-6872
Training Group(s):
Membrane Biology
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Current Research Interests:
Research in Dr. Buhman's laboratory focuses on the process of intestinal lipid absorption and how it regulates whole animal energy balance. Current research in the laboratory addresses the following questions: 1) What is the role of triglyceride synthesis in absorptive cells of the intestine on whole animal energy balance? 2) What is the role of fatty acid catabolism in absorptive cells of the intestine on whole animal energy balance? 3) What is the role of triglyceride storage in lipid droplets during the process of intestinal lipid absorption?
Selected Publications:
Antalis, C.J., Arnold, T., Rasool, T., Lee, B-G., Buhman, K.K. and Siddiqui, R.A. (2009) High ACAT1 expression in estrogen receptor negative basal-like breast cancer cells is associated with LDL-induced proliferation. Breast Cancer Res Treat Oct 23 [Epub ahead of print]
Lee, B-G, Zhu, J, Wolins, N. Cheng J-X, Buhman K.K. (2009) Differential association of Adipophilin and TIP47 proteins with cytoplasmic lipid droplets in mouse enterocytes during dietary fat absorption. Biochim Biophys Acta. 1791(12):1173-80.
Zhu, J., Lee, B-G, Buhman K.K.*, Cheng J-X*. (2009) Direct Observation of a Dynamic Triglyceride Pool in Mouse Enterocytes during Dietary Fat Absorption by Coherent Anti-Stokes Raman Scattering Imaging. Journal of Lipid Research. 50(6):1080-9. * Co-corresponding authors.
Antalis, C.J., Arnold, T., Lee, B-G, Buhman K.K., and Siddiqui, R.A. (2009) Docosahexaenoic acid is a substrate for ACAT1 and inhibits cholesterol ester formation from oleic acid in MCF-10A cells. Prostaglandins, Leukotrienes, and Essential Fatty Acids. 80(2-3):165-71
Chen, H., Wang, H., Jung, Y., Shi, Y., Zhu, J., Buhman, K., Cheng, J-X (2009) A Multimodal Platform for Nonlinear Optical Microscopy and Microspectroscopy. Optics Express. 17(3):1282-1290.
Li, J., Byrne, M. E., Chang, E., Donkin, S.D., Buhman, K.K., Burgess, J. R., and Teegarden, D. (2008) 1alpha,25-dihydroxyvitamin D Hydroxylase in Adipocytes. The Journal of Steroid Biochemistry and Molecular Biology. 112(1-3):122-6.
Siddiqui, S.M.K., Chang, E., Li, J., Burlage, C., Zhou, M., Buhman, K.K., Koser, S., Donkin, S.S., Teegarden, D. (2008) Dietary Intervention with Vitamin D, Calcium and Whey Protein Reduced Fat Mass and Increased Lean Mass in Rats. Nutrition Research. 28(11):783-90.
Borradaile N.M., Buhman K.K., Listenberger L.L., Magee C.J., Morimoto E.T.A., Ory D.S., Schaffer J.E.. (2006) A critical role for eukaryotic elongation factor (eEF) 1A-1 in lipotoxic cell death. Mol Biol Cell. 17(2):770-8.
Tang, Y, Swietlicki E. A., Jiang, S., Buhman, K.K., Davidson, N.O., Burkly, L.C., Levin M.S., Rubin, D. C. (2006) Increased apoptosis and accelerated epithelial migration following inhibition of hedgehog signaling in adaptive small bowel post-resection. Am J. Physiol. Gastrointest. Liver Physiol. 290(6):G1280-1288.
Repa J.J., Buhman K.K., Farese R.V. Jr, Dietschy J.M., Turley S.D. (2004) ACAT2 deficiency limits cholesterol absorption in the cholesterol-fed mouse: Impact on hepatic cholesterol homeostasis. Hepatology 40(5):1088-1097.
Buhman K.K., Wang L.C., Tang Y., Swietlicki E.A., Kennedy, S., Xie, Y., Liu, Z.Y., Burkly L.C., Levin M.S., Rubin D.C., and Davidson N.O. (2004) Inhibition of Hedgehog signaling protects adult mice from diet-induced weight gain. J. Nutrition. 134(11):2979-84.
Xie Y., Nassir F., Luo J., Buhman K., and Davidson N.O. (2003) Intestinal lipoprotein assembly in apobec-1-/- mice reveals subtle alterations in triglyceride secretion coupled with a shift to larger lipoproteins. Am J. Physiol. Gastrointest. Liver Physiol. 285(4):G735-46.
Wilner E.L., Tow B., Buhman K.K., Wilson M., Sanan D.A., Rudel L.L., Farese R.V. Jr. (2003) Deficiency of acyl CoA:cholesterol acyltransferase 2 prevents atherosclerosis in apolipoprotein E-deficient mice. Proc. Natl. Acad. Sci. U S A. 100(3):1262-7.
Newberry E.P., Xie Y., Kennedy S., Han X., Buhman K.K., Luo J., Gross R.W., Davidson N.O. (2003) Decreased hepatic triglyceride accumulation and altered fatty acid ptake in mice with deletion of the liver fatty acid binding protein gene. J. Biol. Chem. 278(51):51664-72.
Buhman K.K., Smith S.J., Stone S.J., Repa J.J., Wong J.S., Knapp F.F. Jr, Burri B.J., Hamilton R. L., Abumrad N.A., Farese R.V. Jr. (2002) DGAT1 is not essential for intestinal triacylglycerol absorption or chylomicron synthesis. J. Biol. Chem. 277(28):25474-9.
Chen H.C., Stone S.J., Zhou P., Buhman K.K., Farese R.V. Jr. (2002) Dissociation of obesity and impaired glucose disposal in mice overexpressing acyl coenzyme A:diacylglycerol acyltransferase 1 in white adipose tissue. Diabetes. 51(11):3189-95.
Buhman, K.K., M. Accad, S. Novak, R.S. Choi, J.S. Wong, R.L. Hamilton, S. Turley, and R.V. Farese, Jr. (2000) Resistance to diet-induced hypercholesterolemia and gallstone formation in ACAT2-deficient mice. Nat Med. 12:1341-7.
Reviews
Buhman K.K., H.C. Chen, R.V. Farese (2001) The enzymes of neutral lipid synthesis. J. Biol. Chem. 276(44):40369-72.
Buhman K.K., M. Accad, R.V. Farese (2000) Mammalian acyl-CoA:cholesterol acyltransferases. Biochim. Biophys. Acta. 529(1-3):42-154.
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